Individual skill
tooluniverse-primer-design
PCR / qPCR primer and oligo design — design forward/reverse primers for a target region (SantaLucia nearest-neighbor thermodynamics), compute melting temperature (Tm) and annealing temperature (Ta), check GC content, and screen an oligo for hairpins and primer-dimers. Use when you need primers for a sequence, want to QC an existing primer pair, or need the Tm of an oligo. Covers the primer-design rules (Tm matching, GC clamp, 3'-end, length) and the tools' constraint quirks.
skills/tooluniverse-primer-design/SKILL.md
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# PCR / qPCR Primer & Oligo Design
Design primers for a target DNA region, get their Tm/Ta, and QC them for the secondary-structure problems that make a PCR fail.
## When to use this
- Design a forward/reverse primer pair to amplify a region of a sequence.
- Compute the Tm / annealing temperature of a primer.
- QC an existing primer pair (GC clamp, 3'-end, hairpins, self/cross dimers, Tm match).
## Step 1 — Design a primer pair
```bash
tu run DNA_primer_design '{"operation":"primer_design",