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tooluniverse-enzyme-kinetics

Enzyme kinetics — Michaelis-Menten Km, Vmax, kcat (turnover), and kcat/Km (catalytic efficiency / specificity constant) from substrate-velocity data, plus inhibition-mechanism analysis (competitive / uncompetitive / non-competitive, Ki). Fits the MM equation by nonlinear regression (and reports Lineweaver-Burk for reference). Use when you have substrate concentrations and initial reaction velocities and need kinetic parameters or to classify an inhibitor. NOT for BRENDA database lookups of published constants (use the BRENDA tools).

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# Enzyme Kinetics (Michaelis-Menten)

Turn **substrate concentration vs initial velocity** data into Km, Vmax, kcat, and catalytic efficiency — and classify an inhibitor's mechanism.

The Michaelis-Menten model: `v = Vmax·[S] / (Km + [S])`.

## When to use this

- You measured initial reaction rates at several substrate concentrations.
- You need Km (substrate affinity), Vmax, kcat (turnover number), or kcat/Km.
- You have ±inhibitor velocity data and want to classify the inhibition mode + Ki.

For *published* kinetic constants (someone else's Km/kcat), use the BRENDA tools or `SABIO_RK_search_reactions` instead — this skill is for analyzing **your own measured** data.
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