Individual skill
pysam
Genomic file toolkit. Read/write SAM/BAM/CRAM alignments, VCF/BCF variants, FASTA/FASTQ sequences, extract regions, calculate coverage, for NGS data processing pipelines.
skills/05-生物信息与基因组学/pysam/SKILL.md
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# Pysam
## Overview
Pysam is a Python module for reading, manipulating, and writing genomic datasets. Read/write SAM/BAM/CRAM alignment files, VCF/BCF variant files, and FASTA/FASTQ sequences with a Pythonic interface to htslib. Query tabix-indexed files, perform pileup analysis for coverage, and execute samtools/bcftools commands.
## When to Use This Skill
This skill should be used when:
- Working with sequencing alignment files (BAM/CRAM)
- Analyzing genetic variants (VCF/BCF)
- Extracting reference sequences or gene regions
- Processing raw sequencing data (FASTQ)
- Calculating coverage or read depth