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flowio

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Parse FCS (Flow Cytometry Standard) files v2.0-3.1. Extract events as NumPy arrays, read metadata/channels, convert to CSV/DataFrame, for flow cytometry data preprocessing.

skills/05-生物信息与基因组学/flowio/SKILL.md

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# FlowIO: Flow Cytometry Standard File Handler

## Overview

FlowIO is a lightweight Python library for reading and writing Flow Cytometry Standard (FCS) files. Parse FCS metadata, extract event data, and create new FCS files with minimal dependencies. The library supports FCS versions 2.0, 3.0, and 3.1, making it ideal for backend services, data pipelines, and basic cytometry file operations.

## When to Use This Skill

This skill should be used when:

- FCS files requiring parsing or metadata extraction
- Flow cytometry data needing conversion to NumPy arrays
- Event data requiring export to FCS format
- Multi-dataset FCS files needing separation
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