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scvelo

Performs RNA velocity analysis with scVelo from spliced and unspliced single-cell RNA counts. Fits deterministic or dynamical models, examines gene phase portraits, builds velocity graphs, estimates relative latent time, and ranks velocity-associated genes. Use for directional trajectory hypotheses and kinetic-model diagnostics alongside Scanpy; velocity alone does not establish cell fate or causal drivers.

skills/scvelo/SKILL.md

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# scVelo RNA Velocity

## When to use

Use for kinetic analysis of aligned spliced/unspliced RNA counts, directional
trajectory hypotheses, gene-level phase portraits, and dynamical latent time.
Use the [model reference](references/velocity_models.md) for assumptions,
transition probabilities, and an optional CellRank handoff.

RNA velocity estimates an expression derivative under a model. Smooth arrows
are not observed cell movement, lineage tracing, causal drivers, or proof of
future fate. The dynamical estimator is not automatically more accurate than
a steady-state estimator on every dataset.
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