Individual skill

nwb-conversion

Converts neuroscience acquisition data to Neurodata Without Borders files with NeuroConv and PyNWB, preserves metadata and timebases, checks evidence-based clock alignment, and produces schema validation, NWB Inspector findings and round-trip checks. Use for NWB conversion and synchronization of planar single-channel two-photon TIFF imaging plus timestamped behavioral position CSV; this skill does not perform spike sorting or claim tested support for arbitrary acquisition formats.

skills/nwb-conversion/SKILL.md

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Nov 9, 2026
License
MIT

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# Validated NWB conversion

## Supported streams

The executable workflow covers two explicit input streams in one session:

| Input | NWB representation | Tested constraints |
| --- | --- | --- |
| Two-photon grayscale multi-page TIFF + frame timestamps CSV | Acquisition `TwoPhotonSeries` named `Imaging` through NeuroConv | One channel, one plane, one 2D image per page, fixed shape and dtype |
| Calibrated position CSV (`time_s,x,y`) | Behavior `Position` / `SpatialSeries` through PyNWB | Coordinates in m, cm or mm; converted to meters without temporal resampling |

Other acquisition readers require their own format-specific tests. In particular, this helper does
not decode SpikeGLX, Open Ephys, multichannel TIFF, volumetric TIFF, compressed video, or pixel-to-world
calibration. Do not rename an arbitrary numeric table to a supported stream.
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