Individual skill
sanger-sequencing
Sanger sequencing workflows — primer design, reaction setup, chromatogram QC, and variant interpretation.
skills/sanger-sequencing/SKILL.md
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## Overview
sanger-sequencing covers the still-essential chain-termination sequencing method: when Sanger
beats NGS (validation, small targets, plasmids, clinical confirmation), primer design, reaction
setup, chromatogram quality assessment, and interpreting variants including heterozygous calls.
Sanger remains the gold standard for validating NGS variants and sequencing short defined
targets — reports of its death are exaggerated.
## When to use
- Validating NGS variants (the classic Sanger confirmation).
- Plasmid/insert verification and colony screening.
- Primer design for sequencing (distinct from PCR primers).
- Chromatogram QC: Phred scores, mixed peaks, dye blobs.