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cell-culture-basics
Mammalian cell culture fundamentals — aseptic technique, passaging, cryopreservation, and contamination control.
skills/cell-culture-basics/SKILL.md
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## Overview
cell-culture-basics covers the essential practices of mammalian cell culture: aseptic technique,
media and supplements, passaging, counting, cryopreservation, and contamination detection and
response. Cell culture is a craft — most failures come from technique drift and unvalidated
assumptions, not from bad luck.
## When to use
- Setting up culture: hood technique, media selection, serum considerations.
- Routine maintenance: feeding schedules, confluency, passaging, splitting ratios.
- Cryopreservation and thawing: DMSO protocols, viability, cell banking.
- Contamination: detection (bacterial, fungal, mycoplasma), response, prevention.
- Cell line authentication: STR profiling, when and how often.