Individual skill
crispr-screen-design
Designing CRISPR screens — library choice, pooled screen workflow, MOI, and hit validation.
skills/crispr-screen-design/SKILL.md
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## Overview
crispr-screen-design covers pooled CRISPR screens (knockout, CRISPRi, CRISPRa): choosing libraries
and modalities, designing the screen for statistical power, executing selection and sequencing,
analyzing with MAGeCK-style tools, and validating hits. Pooled screens are powerful but
unforgiving — design errors (low coverage, wrong MOI, bad selection) are discovered after weeks
of work.
## When to use
- Choosing screen modality: knockout (Cas9) vs CRISPRi vs CRISPRa for the question.
- Library selection: genome-wide vs focused, sgRNA count per gene.
- Screen design: cell number, coverage (cells per sgRNA), MOI, replicates.
- Selection schemes: viability, FACS-based, single-cell readouts.