Individual skill
elisa-assay-design
ELISA development and validation — sandwich vs competitive formats, standard curves, and assay qualification.
skills/elisa-assay-design/SKILL.md
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## Overview
elisa-assay-design covers developing enzyme-linked immunosorbent assays from scratch or
optimizing kits: format selection, antibody pairing, blocking and buffer optimization, standard
curves, and the validation parameters (accuracy, precision, LLOQ/ULOQ) that make an ELISA a
measurement tool rather than a color-change ritual.
## When to use
- Choosing ELISA format: direct, indirect, sandwich, competitive.
- Antibody pair selection and validation for sandwich ELISAs.
- Optimization: coating, blocking, detection antibody, substrate, buffers.
- Standard curves: 4PL/5PL fitting, weighting, acceptance criteria.
- Validation: precision, accuracy, dilutional linearity, spike-recovery, LLOQ/ULOQ.