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flowio

Read, inspect, and write Flow Cytometry Standard (FCS) 2.0, 3.0, and 3.1 files with FlowIO. Use for low-level FCS metadata and channel inspection, NumPy event extraction, multi-dataset files, table export, and FCS 3.1 creation; use FlowKit for compensation, cytometry transforms, gating, or FlowJo workspaces.

plugins/KalarisLabs/research-agent-skills/skills/flowio/SKILL.md

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Nov 6, 2026
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Apache-2.0

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# FlowIO

## Purpose

Use FlowIO as a lightweight, low-level reader and writer for Flow Cytometry
Standard files. Examples in this skill target **FlowIO 1.4.0**, the current
stable release verified on 2026-07-23.

FlowIO is appropriate for:

- Reading FCS 2.0, 3.0, and 3.1 files
- Inspecting HEADER, TEXT, ANALYSIS, and channel metadata
- Retrieving event data as a two-dimensional NumPy array
- Reading legacy files that contain multiple datasets