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scvi-tools

Trains and applies scvi-tools probabilistic deep generative models (scVI, scANVI, totalVI, MultiVI, PeakVI, DestVI, Solo, CellAssign, MrVI and others) on AnnData or MuData single-cell data using PyTorch. Covers batch correction, integration, cell type annotation, probabilistic differential expression, and scRNA-seq, ATAC-seq, CITE-seq, spatial and methylation data. Use when integrating batches or datasets with scVI, annotating cells with scANVI, jointly modeling RNA and protein or ATAC, deconvolving spatial spots, or testing differential expression with uncertainty. For standard preprocessing and clustering pipelines, use scanpy instead.

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# scvi-tools

## Overview

scvi-tools is a comprehensive Python framework for probabilistic models in single-cell genomics. Built on PyTorch and PyTorch Lightning, it provides deep generative models using variational inference for analyzing diverse single-cell data modalities. Current stable release: **scvi-tools 1.4.3** (May 2026).

**Model namespaces matter:** core models (scVI, scANVI, totalVI, MultiVI, PeakVI, AUTOZI, CondSCVI, DestVI, LinearSCVI, AmortizedLDA, JaxSCVI) live under `scvi.model`. Most other models (VeloVI, contrastiveVI, CellAssign, PoissonVI, scBasset, MrVI, MethylVI/MethylANVI, CytoVI, SysVI, Decipher, gimVI, scVIVA, ResolVI, Stereoscope, Solo, totalANVI, DIAGVI) live under `scvi.external`. The reference files specify the correct namespace per model.

## When to Use This Skill

Use this skill when:
- Analyzing single-cell RNA-seq data (dimensionality reduction, batch correction, integration)
- Working with single-cell ATAC-seq or chromatin accessibility data
- Integrating multimodal data (CITE-seq, multiome, paired/unpaired datasets)